Short Communication Dna-binding Properties and Cytotoxicity of Extended Aromatic Bisamidines in Breast Cancer Mcf-7 Cells
نویسندگان
چکیده
The DNA binding properties of three novel extended aromatic bisamidines (1–3) possessing different dicationic terminal side chains were studied. Data from the ethidium displacement assay showed that bisamidines 1–3 have significant affinity for DNA. We studied the cytotoxic activity of bisamidines 1–3 and Hoechst 33258 in the cultured breast cancer MCF-7 cells. These data show that in broad terms the cytotoxic potency of bisamidines 1–3 in the cultured breast cancer MCF-7 cells decreases with the size of the alkyl group substituent (cyclopropyl > isopropyl > cyclopentyl), in accord with their increases in DNA affinity, as shown by the binding constant values. The bisamidines 1–3 showed comparable antitumor activity to Hoechst 33258.
منابع مشابه
DNA-binding properties and cytotoxicity of extended aromatic bisamidines in breast cancer MCF-7 cells.
The DNA binding properties of three novel extended aromatic bisamidines (1-3) possessing different dicationic terminal side chains were studied. Data from the ethidium displacement assay showed that bisamidines 1-3 have significant affinity for DNA. We studied the cytotoxic activity of bisamidines 1-3 and Hoechst 33258 in the cultured breast cancer MCF-7 cells. These data show that in broad ter...
متن کاملEvaluation of Anti-oxidant and Anti-cancer Properties of Silver Nanoparticles Synthesized by Apigenin toward Breast Cancer MCF-7 Cell Line
Introduction: Cancer is one of the most common diseases in the modern societies, which results from the non-stop growth of cells in the body. Due to the advancement of nanobiotechnology, highly effective herbal metabolites can be used to treat cancer. Apigenin is a natural flavonoid that is found in abundance in fruits, vegetables and herbs. The purpose of this study was to investigate the anti...
متن کاملCytotoxicity Effect of Cladribine on the MCF-7 Human Breast Cancer Cell Line
Cladribine, an analogue of deoxyadenosine, is highly toxic for both non-dividing and proliferating cells and has shown activity in the treatment of several malignancies. Therefore, the aim of the present study is to investigate the cytotoxicity effect of cladribine (2-CdA) on the breast cancer cell line, MCF-7 (estrogen receptor positive, ER+). MTT assay, annexin V-Fluorescein/PI and Hoechst 33...
متن کاملStudy of Cytotoxic Effects of Saffron in MCF-7 Cells
Current therapies for breast cancer are often limited by short-term efficacy due to the emergence of drug resistance. There has been increased interest in the use of naturally occurring compounds with chemopreventive and chemotherapeutic effects in the treatment of cancers. Saffron, dry stigmas Crocus sativus L., used in Iran as a spice, is known for its anticancer properties. In...
متن کاملGrowth Inhibition and Apoptosis Induction of Salvia chloroleuca on MCF-7 Breast Cancer Cell Line
Fragrant species of the genus Salvia have been attributed many medicinal properties, which include anticancer activity. In the present study, cytotoxic properties of total methanol extract of Salvia chloroleuca Rech. f. & Aellen and its fractions were investigated on MCF-7, a breast carcinoma cell line. Malignant and non-malignant cells were cultured in RPMI medium and incubated with different ...
متن کامل